Genome-wide methylation sequencing (WGBS) has been a cornerstone in epigenetics research, enabling the exploration of DNA methylation patterns across the genome. However, traditional WGBS has faced limitations, especially in handling low-input samples. In response, novel variants such as PBAT-WGBS, T-WGBS, single-cell WGBS, and nanoporous WGBS have emerged. This review...